Patrick Sobetzko

Patrick Sobetzko

Dr. Patrick Sobetzko

SYNMIKRO Research Center
Chromosome Biology
Hans-Meerwein-Straße, 35032 Marburg
+49-6421 28 22261

Research Area

Bacterial chromosomes are more than a collection of genes, randomly scattered on a circular molecule. Chromosomal DNA sequences contain multiple layers of information coding for spatio-temporal structural and regulatory organisation. Our group focuses on the impact of DNA sequence organisation on regulatory processes from single bases over small DNA streches, forming structural elements, to gene arrangements and the overall global chromosome design. We study the impact of DNA modifications using systematic genome editing and DNA sequence library analysis. Moreover, we develop and apply cutting edge CRISPR-Cas9 genome editing, optogenetics, and genome assembly technologies in combination with high-throughput omics data analysis.


synthetic microbiology
microbial genetics
DNA 3D topology
DNA supercoiling
promoter studies
artificial regulatory circuits
CRISPR Cas9 and recombinase-based genome editing tool development
synthetic chromosomes
comparative genomics and transcriptomics

Recent Publications, Last 3 Years

1. Klein CA, Emde L, Kuijpers A, Sobetzko P. (2019) MoCloFlex: A Modular Yet Flexible Cloning System. Front Bioeng Biotechnol. 2019 Oct 17;7:271. doi: 10.3389/fbioe.2019.00271. eCollection 2019.

2. Sobetzko P, Jelonek L, Strickert M, Han W, Goesmann A, Waldminghaus T. (2016) DistAMo: A Web-Based Tool to Characterize DNA-Motif Distribution on Bacterial Chromosomes. Front Microbiol. 2016 Mar 11;7:283. doi: 10.3389/fmicb.2016.00283. eCollection 2016.

3. Sobetzko P. (2016) Transcription-coupled DNA supercoiling dictates the chromosomal arrangement of bacterial genes. Nucleic Acids Res. 2016 Feb 29;44(4):1514-24. doi: 10.1093/nar/gkw007. Epub 2016 Jan 17.

SYNMIKRO Young Researchers Groups

Almost all scientific members of SYNMIKRO are actively involved in DFG’s Collaborative Research Centers (Sonderforschungsbereiche), Research Training Groups (Graduiertenkollegs), or other Cooperative Research projects. Alongside performing adventurous experiments, and reporting excellent science, SYNMIKRO substantially promotes potential Young Research Group Leaders by constantly keeping its doors open to welcome and support Young Researchers planning to set up an Independent Research Group.
Our Young Research Groups