Max Planck Institute for Terrestrial Microbiology
Dynamic Control of Metabolic Networks
Karl-von-Frisch-Straße 16, 35043 Marburg
+49-6421 28 21624
Our research goal is to uncover the regulatory network that controls metabolism. This network is largely unexplored compared to the well-mapped stoichiometry of metabolism. A major barrier is that we don’t know which of the many metabolites in a cell interact with enzymes and regulatory proteins, and that we don’t understand the regulatory role of these interactions. To address these problems, our group combines molecular biology methods (e.g. CRISPR perturbations), multi-omics analysis and computational methods. Metabolomics methods are especially important, and we develop methods for measuring hundreds of metabolites using state-of-the-art mass spectrometry technology.
CRISPR genome editing
1. Lempp M, Farke N, Kuntz M, Freibert S, Lill R, Link H (2019). Systematic identification of metabolites controlling gene expression in E. coli. Nature Communications 10, 4463.
2. Sander T, Wang CY, Glatter T, Link H (2019). CRISPRi-based downregulation of transcriptional feedback improves growth and metabolism of arginine overproducing E. coli. ACS Synthetic Biology 8, 1983-1990.
3. Sander T, Farke N, Diehl C, Kuntz M, Glatter T, Link H (2019). Allosteric feedback inhibition enables robust amino acid biosynthesis in E. coli by enforcing enzyme overabundance. Cell Systems 8, 66-75.