Andreas Diepold

Research Area

The Diepold lab studies the molecular function and regulation of the type III secretion system, a nano-syringe used by bacteria to manipulate eukaryotic host cells. In particular, we want to understand how protein secretion is activated, precisely adapted and stopped by external signals, and how these mechanisms enable bacteria to successfully establish an infection. We found that function and regulation of the T3SS require active remodelling of the system, and our lab follows these dynamic processes in live bacteria, in real time, from the single-molecule level up to cellular host-pathogen interactions. Using synthetic biology approaches, we aim to control and apply the T3SS for biotech and healthcare.

Expertise

fluorescence microscopy
protein dynamics
microbe-host interactions
optogenetics
membrane transport
infection biology
microbial genetics
bacterial cell biology

Recent Publications, Last 3 Years

1. Unni R, Pintor KP, Diepold A, Unterweger D (2022) Presence and absence of type VI secretion systems in bacteria. Microbiology (in press)

2. Gurung JM, Amer AA, Chen S, Diepold A, Francis MS. (2022) Type III secretion assembly in Yersinia pseudotuberculosis is reliant upon an authentic N-terminal YscX secretor domain. Mol. Microbiol. doi: 10.1111/mmi.14880 (in press)

3. Linder F & Diepold A. (2022) Optogenetics in Bacteria – Applications and Opportunities. FEMS Microbiol Rev. fuab055

https://www.mpi-marburg.mpg.de/diepold