Protein Biochemistry and Spectroscopy

Protein Biochemistry and Spectroscopy

Dr. Oliver Stehling


Center for Synthetic Microbiology
Medicine
Karl-von-Frisch-Straße 14, 35032 Marburg
+49 6421 28-64044
stehling@staff.uni-marburg.de


Prof. Dr. Roland Lill

Center for Synthetic Microbiology
Medicine
Karl-von-Frisch-Straße 14, 35032 Marburg
+49 6421 28-66449
lill@staff.uni-marburg.de

Description

The core facility for protein spectroscopy in the Institute of Cytobiology provides high quality spectrometers, anaerobic facilities, stopped-flow and chromatography equipment. Applications and expertise are focussed on proteins with cofactors and on metalloproteins for which we have extensive experience (see publications below). We support all students and faculty of the Philipps University and their collaboration partners outside our university.

Major instrumentation

  • Jasco FP-6300 Fluorimetry

  • Jasco J-815 CD-Spectrometry

  • Biologic SFM-20 (Stopped flow kinetics)

  • Bruker EMX-6/1 (EPR spectroscopy)

  • Nanotemper Prometheus NT.48, Differential Scanning Fluorimeter (DSF)

  • Nanotemper Monolith NT.115, Microscale-Thermophoresis (MST)

  • Pall ForteBio Octet K2, Biolayer Interferometry (BLI)

  • 4 Anaerobic tents (one with ÄKTA for anaerobic protein purification)

  • Analytical HPLC (Waters/Raytest) with diode array detector

  • 4 Tecan multiplate readers (absorption, fluorescence, luminescence, fluorescence polarisation)

  • Becton Dickinso FACSCanto II flow cytometer

  • Berthold Sirius L – tube luminometer

more details under:
https://www.uni-marburg.de/en/fb20/departments/cyto/core-facilities/core-facility-1

SYNMIKRO Young Researchers Groups

Almost all scientific members of SYNMIKRO are actively involved in DFG’s Collaborative Research Centers (Sonderforschungsbereiche), Research Training Groups (Graduiertenkollegs), or other Cooperative Research projects. Alongside performing adventurous experiments, and reporting excellent science, SYNMIKRO substantially promotes potential Young Research Group Leaders by constantly keeping its doors open to welcome and support Young Researchers planning to set up an Independent Research Group.
Our Young Research Groups